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KMID : 0861020030180020179
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Korea Journal of Herbology
2003 Volume.18 No. 2 p.179 ~ p.188
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Effects of Acanthopanacis senticosi Radix on anti-CD40- and recombinant interleukin-4-induced cytokine production in murine B-cells
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Sung Il-Chang
Kim Hyung-Hwan
Ahn Duk-Kyun
Lee Yong-Sup
Seo Young-Bae
Choi Ho-Young
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Abstract
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Objectives : In order to study the anti-allegy effect of water extract of Acanthopanacis senticosi Radix (ASR) on the B-cells from healthy Balblc mice.
Methods : The cytotoxicity of ASR was measured with the murine normal lung fibroblast cells by modified SRB assay. And the murine splenic B-cells was stimulated with anti-CD40 mAb and rIL4. The various cytokines related with allergy were measured by flow-cytometry and by RT-PCR with electophoresis.
Results : The anti-allegy effects to ASR were identified and observed. The cytotoxicity of ASR on mouse lung fibroblast cells showed no significant activities. ASR had inhibitory effect on CD23+, CD69+, and IgE expression by ASR with anti-CD40 mAb plus rL-4-stimulated murine splenic B-cells. ASR had inhibitory effect on cytokines (E-lb, IL-4, IL-6, IL-10, TNF-a, TGF-81, INF-Y) and transcript expression and IgE production by ASR with anti-CD40 mAb plus rIL-4-stimulated murine splenic B-cells.
Conclusion : We concluded that ASR showed anti-allegy effect on murine splenic B-cells.
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